Fig. 6

A CDNA sequencing analysis revealed that the c.575G > T nucleotide mutation was introduced into phage -PDHB-mut. B PDHB mRNA expression in wild-type transfected cells and in mutant transfected cells (qPCR). C PDH protein expression in wild-type transfected cells and in mutant transfected cells. D PDH activity were measured in wild-type transfected cells and in mutant transfected cells. The data is representative of three independent experiments in qPCR, western blot, and PDH activity measurement. Data were presented as mean ± SD. Statistical significance was analyzed using two-way ANOVA with Dunnett’s multiple comparisons test. ***: compared to wild-type transfected cells, P < 0.001