Fig. 4

A Tolerance landscape of RIPK4 protein variants. The color-coded gradient represents the degree of predicted tolerance to amino acid substitutions along the RIPK4 protein sequence, with blue indicating high tolerance and red indicating low tolerance. Notable substitutions, p.E452K and p.T520S, are highlighted, demonstrating regions of potential functional impact. The gradient scale on the left denotes the transition from tolerant (top) to intolerant (bottom) regions. B Comparison of protein structures at the mutation site between the wild type and mutant type